TitleDielectrophoresis-based discrimination of bacteria at the strain level based on their surface properties.
Publication TypeJournal Article
Year of Publication2013
AuthorsBraff WA, Willner D, Hugenholtz P, Rabaey K, Buie CR
JournalPloS one
Volume8
Paginatione76751
Date Published2013
ISSN1932-6203
AbstractInsulator-based dielectrophoresis can be used to manipulate biological particles, but has thus far found limited practical applications due to low sensitivity. We present linear sweep three-dimensional insulator-based dielectrophoresis as a considerably more sensitive approach for strain-level discrimination bacteria. In this work, linear sweep three-dimensional insulator-based dielectrophoresis was performed on Pseudomonas aeruginosa PA14 along with six isogenic mutants as well as Streptococcus mitis SF100 and PS344. Strain-level discrimination was achieved between these clinically important pathogens with applied electric fields below 10 V/mm. This low voltage, high sensitivity technique has potential applications in clinical diagnostics as well as microbial physiology research.
DOI10.1371/journal.pone.0076751

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