test tubes are analysed

Precision Profiling

Swiftly discover the composition of your samples with our amplicon-based community profiling service. Our extensive range of primers means you control the specificity of your profile, so you can focus just on the organisms that interest you.

Recent advancements in Next Generation Sequencing technology now allow for the rapid generation and cost-effective sequencing of samples in great depth. Using this technology, we are able to amplify portions of genes of interest that contain hypervariable regions that can be used to distinguish and identify different organisms from a culture-independent sample.

Our service offers a large range of standard primer sets that are appropriate to use to identify Bacteria, Archaea, Eukaryotes, Plants and Fungi. In addition, we can use custom primer sets to specifically investigate your target of interest.

Our aim is to help you obtain the best scientific outcome for your study with flexible options to suit each project. Using our online sample submission centre, as a client you are able to lodge up to 380 samples per sequencing run at your convenience. Your samples are able to enter our workflow at any point depending on your preference.

Sample Requirements

Sample Number: 1 - 380 samples per job. Contact us for additional multiplexing options.

Sample Format: We accept raw samples (e.g. soil, water, swabs), extracted genomic DNA, PCR products and pooled amplicon samples.

Sample Quantity: Optimal inputs are 50 - 100mg for raw samples and 20uL of sample at approximately 5ng/ul of template DNA for extracted DNA, however we are capable of sequencing samples with quantities outside these ranges. Contact us for more information.

Data Return: A guaranteed minimum of 10,000 reads per sample will be returned as FASTQ files. In addition, each sample will generate an OTU table that can be viewed in Excel.

Costs: Costs for profiling services depend on the range of services you require for your project. Contact us so that we can prepare a quote tailored to your specific needs.

Sample Requirements
We offer a range of molecular biological assistance including:
  • DNA extractions from raw samples
  • qPCR
  • Quantification and QC of submitted DNA prior to amplicon production

Our DNA extractions are performed on the Maxwell automated DNA extraction system producing high quality DNA that is able to be used in qPCR (16S rRNA) and amplicon generation.

qPCR at our centre is performed using the ViiA™ 7 Real-Time PCR System (Life Technologies) to determine the microbial biomass based on copy number.

If your laboratory lacks the facilities, we are able to perform a range of lesser costed QC checks on your submitted DNA prior to amplicon generation. These include gel analysis, PCR and accurate quantification.

Our amplicon based community analysis assay is able to be applied to gDNA from a wide range of samples including environmental samples, tissue samples, food samples and industrial products.

Our currently held primer sets include primers which cover:

  • V1 - V3, V3 - V4, V5 - V8 or V6 - V8 hypervariable regions of the 16S rRNA gene
  • V4 - V5 hypervariable regions of the 18S rRNA gene
  • ITS2 of fungal genes from 5.8S to 28S

If you have an alternate region or gene of interest we are able to order custom primers to be used for your samples.

Our amplicon sequencing service has been optimized to allow automated analysis of your resulting sequence data through our standard data analysis pipelines. We provide ready-to-use data, in Excel readable format, which will outline the organisms identified in your samples and their relative abundance within the community. Our automated data processing pipeline has been developed by leading researchers at ACE and accesses the most up to date and comprehensive databases available.

Additional bioinformatics support for more sophisticated data analysis is available upon request and charged at an hourly rate. This service allows you to work one on one with an allocated data analyst to explore your data in more depth.

DNA deep sequencing

Deeper Insights

Go beyond amplicon profiles. Our metagenomic and metatranscriptomic services allow you to explore your samples to new depths. Choose from shallow, deep or ultra-deep sequencing, and gain a more comprehensive picture of your community.

Shotgun metagenomic sequencing allows researchers to comprehensively sequence all of the genes, in all of the organisms, present in a complex uncultured sample. This data is able to be compiled to evaluate bacterial diversity, proposed functionality and comparative abundance within your sample. Unlike previous methods, the use of NGS allows researchers to combine many samples in a single sequencing run, sequencing thousands of organisms in parallel whilst maintaining high coverage per sample.

Shotgun metagenomic sequencing is available using a range of Illumina based library preps dependant on the amount of starting material available and client preference. Currently clients have access to Nextera XT, Nextera, TruSeq Nano and TruSeq PCR Free library preps.

Your libraries are available to be run as shallow, deep or ultra deep depending on the diversity of your population or the level of coverage required. Additional services such as transcriptome sequencing (identifying and quantifying expressed transcripts) are available if required.

Please note that our shotgun sequencing service returns data as sequence files only. If you require bioinformatics support we can refer you to specialist centres that offer these services and can work with you to analyse your data.

Sample Requirements

Details: We have a number of Illumina shotgun metagenomics library preparation systems available depending on your specific requirements. If you are uncertain which library prep would best suit your experimentation we suggest a review of material provided on the Illumina website. This site will provide you with a comprehensive explanation of each method (www.illumina.com)

Sample Quantity: Required quantities for library preparation kits given below:

Nextera XT: Minimum concentration 0.25ng/ul, minimum volume 15ul.

Nextera: Minimum concentration 2.5ng/ul, minimum volume 30ul.

TruSeq Nano: Minimum concentration 2.5ng/ul, minimum volume 50ul.

Data Return: Returned as FASTQ files only. Contact us for additional bioinformatic analysis options.

Costs: Costs for metagenomic services depend on the range of services you require for your project. Contact us so that we can prepare a quote tailored to your specific needs.

Sample Requirements
ACE logo

Powering Research

Have confidence in your results. As part of the Australian Centre for Ecogenomics, we understand first-hand the requirement for quality genomic sequencing services. It forms the basis for not only our clients, but our own world class research.

ACE Sequencing Service is part of the Australian Centre for Ecogenomics, a renowned and leading research group in the field of ecogenomics and associated bioinformatics within the University of Queensland. Our co-directors, Professor Phil Hugenholtz and Professor Gene Tyson, have active research groups in a variety of experimental settings from the human microbiome to bacterial mediated methane emissions in the melting permafrost of Sweden. As co-founder and curator of the widely used Greengenes 16S rRNA gene sequence database, Professor Hugenholtz maintains an active interest and has access to the most up to date classification databases and programs. Further information about the dynamic research at the Australian Centre for Ecogenomics can be found at ecogenomic.org.

ACE Sequencing applies a rigorous, validated standard operating procedure for the processing and analysis of your samples. However, being at the nexus of active research projects and service provision, our sequencing centre continues to apply sample preparation improvements and implements sequencing advancements, making the outcome of your sample processing the highest quality, the most comprehensive, the most scientifically valid, the most cost effective and the fastest that it can be.

We're here to help. Discuss your project requirements with us today!

Contact Information

Phone contact:
Nicola Angel
Sequencing Manager
+61 7 3365 4006
Email contact:
General enquiries
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Last updated 19 June 2016
Last reviewed 24 May 2016


Australian Centre for Ecogenomics
Level 5, Molecular Biosciences Bldg
University of Queensland
Brisbane, Australia

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